Demonstration of extracellular immunoproteins in formalin-fixed renal biopsy specimens

Diagnostic value of direct immunofluorescence of mucosal and skin biopsy specimens on formalin-fixed paraffin-embedded biopsy specimens compared to specimens placed in normal saline as the gold standard method in the diagnosis of a group of autoimmun

Background and Aim: Direct immunofluorescence (DIF) is represented as a gold standard method in diagnosis of autoimmune blistering dermatoses. Normal saline, liquid nitrogen and michel’ solution are a widely accepted media for preserving biopsy samples of skin or mucosa before DIF examination. Occasionally clinicians put the biopsy specimen taken for DIF in formalin 10%, occasionally clinicians...

Plastination of Old Formalin-fixed Specimens

This paper presents an alternative approach to the study of gross anatomy which may be pursued in Medical Schools using plastination techniques. Specimens fixed with formalin and alcohol-based techniques were utilized as the source of specimens to be plastinated; the reason for this choice was a result of the decreased organ availability in Italy due to religion and restrictive laws. The proced...

Demonstration of immunoproteins in araldite-embedded tissues.

Percutaneous renal biopsy specimens in stone formers.

A series of renal biopsy specimens taken at the time of percutaneous nephrolithotomy were investigated for the presence and location of foci of microcalcification. Calcium was found in 18 of 25 (72%) of biopsy specimens from stone formers and in only seven of 30 (23%) of control biopsy specimens. This may indicate defective intrarenal handling of calcium as plasma calcium concentration was norm...

Autophagy by podocytes in renal biopsy specimens.

Autophagy, a process of bulk protein degradation and organelle turnover, is induced under starvation conditions in yeast and plays an important role in developmental processes and human diseases. Autophagy is a nonapoptotic form of programmed cell death. Disturbances in autophagy and programmed cell death may lead to cancer and several degenerative diseases in humans. During the routine ultrast...